MODIFIED CLOSED CIRCULAR DNA (CCDNA) CONTAINING SYMMETRIC MODIFIED INVERTED END REPEATED SEQUENCES Russian patent published in 2024 - IPC C12N15/63 C12N15/85 C12N15/86 A61P7/00 A61P35/00 A61P25/00 

FIELD: biotechnology.

SUBSTANCE: described is a group of inventions comprising a non-viral capsid-free DNA vector with covalently closed ends (ccDNA vector) for delivering nucleic acid, a ccDNA expression construct which codes ccDNA, a host cell for producing the ccDNA expression construct, method of producing a ccDNA vector, using a composition comprising a ccDNA vector for treating, preventing, relieving, monitoring or diagnosing a disease or disorder in a subject, a method of delivering a therapeutic protein to a subject, a kit for delivering a nucleic acid and a kit for producing a ccDNA vector. In one embodiment, the ccDNA vector comprises a nucleic acid sequence flanked by two symmetric ITRs which are not wild-type ITRs.

EFFECT: invention extends the range of means for delivering nucleic acid.

56 cl, 29 dwg, 7 tbl, 9 ex