FIELD: biotechnology.
SUBSTANCE: invention relates to genetic engineering. Methods of synthetic synthesis and cell-free synthesis of DNA vectors with closed ends (for example, ceDNA vectors), having a linear and continuous structure, for delivery and expression of a transgene are proposed. Present invention relates to an in vitro process for producing closed-ended DNA vectors, corresponding products of DNA vectors obtained using said methods, and their use, as well as to oligonucleotides and kits used in the method according to the invention.
EFFECT: obtained DNA vectors do not have undesirable side effects due to contaminants introduced during the production process in cell lines.
90 cl, 18 dwg, 11 tbl, 8 ex
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