FIELD: medical microbiology.
SUBSTANCE: described is a method of detecting the Classical type tcpA gene in V. cholerae El Tor by real-time PCR, involving extraction of DNA from the strain under study, PCR with a pair of primers with subsequent consideration of the amplification reaction.
EFFECT: development of a simple, fast and reliable method of detecting the Classical type tcpA gene of the Vibrio cholerae using real-time PCR.
3 cl, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR MOLECULAR TYPING FOR TYPICAL (O1) AND ATYPICAL (RO) NON-TOXIGENIC V. CHOLERAE EL TOR STRAINS USING REAL-TIME PCR | 2022 |
|
RU2792156C1 |
| METHOD FOR DETECTION OF TOXIGENIC STRAINS 01 VIBRIO CHOLERAE "POSTGAITIAN" LINE BY PCR IN REAL TIME | 2021 |
|
RU2766192C1 |
| METHOD OF DETECTING COLD SHOCK GENE CSH1 IN STRAINS OF VIBRIO CHOLERAE O1 AND NEO1/NEO139 BY LOOP ISOTHERMAL AMPLIFICATION (LAMP) | 2023 |
|
RU2819101C1 |
| METHOD FOR DETECTING THE CSH 1 COLD SHOCK GENE IN VIBRIO CHOLERAE STRAINS USING REAL-TIME PCR | 2021 |
|
RU2783023C1 |
| METHOD FOR IDENTIFICATION OF VIBRIO-CHLERAE O1 STRAINS, DETERMINATION OF THEIR TOXIGENICITY AND BIOVAR WITH DIFFERENTIATION OF EL TOR BIOVAR ON TYPICAL AND GENETICALLY CHANGED VERSIONS BY MULTIPLEX POLYMERASE CHAIN REACTION AND TEST SYSTEM FOR IMPLEMENTATION THEREOF TAKING INTO ACCOUNT RESULTS IN REAL TIME MODE | 2019 |
|
RU2732448C1 |
| METHOD FOR DETECTING TOXIGENIC STRAINS OF O1 VIBRIO CHOLERAE OF "HAITIAN" GROUP BY PCR IN REAL TIME ON END POINT | 2019 |
|
RU2729218C1 |
| METHOD FOR ASSESSING INTRASPECIFIC COMPETITION OF TOXIGENIC AND NON-TOXIGENIC STRAINS OF VIBRIO CHOLERAE O1 AND O139 SEROGROUPS USING REAL-TIME PCR | 2023 |
|
RU2806564C1 |
| METHOD FOR DETECTING VIABLE VIBRIO CHOLERAE 01 SEROGROUP OF BIOVARS CLASSICAL AND El Tor IN THE ENVIRONMENT USING BACTERIOPHAGE M3 USING QUANTITATIVE PCR METHOD | 2023 |
|
RU2808577C1 |
| METHOD FOR MOLECULAR-GENETIC INTRASPECIFIC TYPING OF TOXYGENIC VIBRIO CHOLERAE O1 ELTOR STRAINS | 2016 |
|
RU2627193C1 |
| METHOD FOR IDENTIFYING TOXIGENIC V CHOLERAE O1 STRAINS, DETERMINING ITS BIOVAR AND DIFFERENTIATING BIOVAR ELTOR STRAINS BY TYPICAL AND CHANGED BY MULTIPLEX POLYMERASE CHAIN REACTION, AND TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2011 |
|
RU2458141C1 |
