METHOD FOR IDENTIFYING TOXIGENIC V CHOLERAE O1 STRAINS, DETERMINING ITS BIOVAR AND DIFFERENTIATING BIOVAR ELTOR STRAINS BY TYPICAL AND CHANGED BY MULTIPLEX POLYMERASE CHAIN REACTION, AND TEST SYSTEM FOR IMPLEMENTATION THEREOF Russian patent published in 2012 - IPC C12Q1/68 C12Q1/02 C12N15/11 

FIELD: medicine.

SUBSTANCE: invention concerns identifying toxigenic Vibrio cholerae O1 strains, determining their biovars and differentiating V.cholerae eltor biovars by typical isolates and changed versions. According to the invention, the multiplex polymerase chain reaction (PCR) is conducted in a single step in two reaction mixtures wherein each mixture contains a specially selected combination of primers: one - to the genes rfbO1, cas3 and ctxB^class another - to the genes rfbO1, rtxC and ctxB^Eltor. A test system for implementing the method comprises DNA recovery components, PCR components and result analysis components. The PCR components contain: the 10-fold buffer solution, pH 8.4, mineral oil, deionised sterile water, two positive controls, enzyme Taq-polymerase, mixed dNTP, mixed primers No.1 - rfbO1-F - rjbO1-R, cas3-F - cas3-R, ctxB^class-F- ctxB^class and mixed primers No.2 - rfbO1-F - rfbO1-R, rtxC-F - rtxC-R, ctxB^Eltor-F - ctxB^Eltor-R.

EFFECT: invention enables fast and reliable detection of serogroup cholera vibrios O1 biovar, detecting their toxigenicity and differentiating the detected toxigenic Vcholerae eltor biovar strains by typical isolates and changed versions.

3 cl, 2 dwg, 3 tbl, 1 ex