METHOD OF PRODUCING LIQUID ENRICHED NUTRIENT MEDIUM FOR CULTURING LEPTOSPIRA Russian patent published in 2025 - IPC C12N1/20 C12N1/38 

FIELD: biotechnology.

SUBSTANCE: invention represents a method for preparing a liquid enriched nutrient medium for leptospira cultivation involving mixing the following components: sodium chloride – 0.5 g; dry enzymatic peptone – 1.0 g; thiamine – 0.005 g in a container, adding 12.5 ml of Tween-80 to the mixture and adding the ready working solution of the phosphate-buffer mixture in distilled water to 1.0 l, boiling until all the ingredients are completely dissolved, the pH of medium is 7.4±0.2, and dispensed into graduated flasks, followed by sealing and sterilization at temperature 115 °C for 15 minutes, cooling down to temperature 56 °C, then in each bottle with a sterile bacteriological pipette, normal horse serum for bacteriological nutrient media liquid is added in amount of 10 ml/l and the medium is poured into sterile bacteriological test tubes.

EFFECT: invention enables to develop a liquid enriched culture medium for culturing leptospira with high growth properties with respect to leptospirosis agents and a simple manufacturing technique.

1 cl, 1 tbl, 3 ex