FIELD: analytical biochemistry. SUBSTANCE: method involves the determination of phonosignal, and after enzyme addition continuous gaseous extraction of product is carried out during the enzymatic reaction course by passing of constant gas flow over incubation mixture. Increment of volatile product concentration is recorded in the gaseous phase by time, and activity is measured by neutralized meaning of this value. Method can be used in medicinal biochemistry, in research and clinical-biochemical laboratories. EFFECT: increased precision and reliability of esterase activity of proteolytic enzymes. 3 dwg, 1 tbl
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