METHOD OF DETERMINATION OF PHAGOSOMES NUMBER IN PHAGOCYTES Russian patent published in 1995 - IPC

FIELD: experimental biology and medicine. SUBSTANCE: crushed unstained and stained phagocytes were examined. Lysosomes, phagosomes and phagolysosomes were distributed by a single layer. Number of bacteria stained with green color were counted in unstained preparation (sum of phagosomes and phagolysosomes). Number of bacteria stained with red color were counted in stained preparation (phagolysosomes). Measurement is carried out per 50-100 phagocytes by subtraction of bacteria number stained with green color in the first preparation bacteria number stained with red color in the second preparation. Difference obtained indicates the number of phagosomes and the real percent content of phagolysosomes. EFFECT: improved image quality.