METHOD FOR PRODUCTION OF CATALASE FROM ERYTHROCYTES OF HUMAN BLOOD Russian patent published in 1995 - IPC

Abstract RU 2033170 C1

FIELD: biochemistry, particularly enzymology. SUBSTANCE: method involves washing erythrocytes of donor blood with physical solution and centrifuging with simultaneous precipitation of hemoglobin, and stabilizing the catalase with a mixture of chloroform and propanol in a ratio of 5:3. The supernatant produced by centrifuging is treated with an equal volume of water and ammonium sulfide is dissolved at the rate of 535-540 g per 1 l of extract until salt is fully dissolved. The formed sediment is again centrifuged. EFFECT: higher intensity of method.

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RU 2 033 170 C1

Authors

Blagorodov S.G.

Svechnikova L.V.

Shepelev A.P.

Sazykina O.V.

Dates

1995-04-20Published

1989-06-07Filed