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2 016 899

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C1

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IPC

(21) (22)

Application

4946819/13, 1991-06-19

(22)

Actual filing date

1991-06-19

(45)

Published

1994-07-30

(72)

Inventor

Stekol'Nikov L.I.Ryl'Tsev V.V.Vinogradova M.N.Bychkov B.I.

(73)

Holder

Nauchno-Issledovatel'Skij Institut Tekstil'Nykh Materialov

METHOD OF ALKALINE PHOSPHATASE PREPARING Russian patent published in 1994 - IPC

Abstract RU 2016899 C1

FIELD: enzymology. SUBSTANCE: cattle spleen is homogenized in water at pH 0.5-0.9, mixture is centrifuged, supernatant is desalted out with sodium chloride at 2-6 C (10-12% of solution volume), and active fraction is dissolved in water (0.1-0.2 water volume to the raw mass). Desalting is carried out by dialysis following by purification of the end product by precipitation and reprecipitation with 4-6 volumes of ethanol at 0-4 C and drying. EFFECT: improved method of enzyme preparing. 1 tbl

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RU 2 016 899 C1

Authors

Stekol'Nikov L.I.

Ryl'Tsev V.V.

Vinogradova M.N.

Bychkov B.I.

Dates

1994-07-30—Published

1991-06-19—Filed

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