FIELD: medicinal biochemistry. SUBSTANCE: extraction of enzymes - superoxide dismutase, catalase, carbonic anhydrase from lysized erythrocytes is carried out with a mixture of halogen-containing hydrocarbon and ethanol. Methylene chloride is used as halogen-containing hydrocarbon at its volume concentration in mixture is 20-50%. Extraction is carried out at stirring at the rate 1200-1500 rev/min for 1-1.5 hr at 0-4 C. EFFECT: increased yield and selectivity of enzyme extraction, simplified method, decreased toxicity of labour condition. 1 tbl
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Authors
Dates
1996-03-27—Published
1992-06-16—Filed