FIELD: medicine. SUBSTANCE: after action with hematoxylin on histosection staining is carried out with 0.1% alcyane-blue prepared on acetate buffer, pH 4.4, for 20-45 min at 37 C in thermostat following by additional staining with eosin at the room temperature. Method ensures to differentiate cartilage and bone interrelation boundary. EFFECT: improved method of staining.
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