FIELD: medicine.
SUBSTANCE: tissue sections are incubated simultaneously with mixed primary and secondary antibodies. Primarily, the primary antibodies are applied on the tissues, and in 15 seconds of the incubation process - the secondary antibodies without washing with a buffer. It is followed with combined incubation for 4 minutes. A substrate-chromogen solution for the incubation is prepared immediately before the application, the sections are washed with a buffer solution and distilled water heated to temperature 37°C.
EFFECT: method allows fast and precise tumour histogenesis, detection of tumour cells within resection boundaries.
6 dwg, 2 tbl, 6 ex
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