FIELD: medicine. SUBSTANCE: method involves diluting plasma separated from cellular sediment to 15-20 times lower concentration, introducing into a spectrophotometer cuvette, adding tris-HCl-buffer heated up to 28-30 C and then Boc-Ala-O- Np substrate solved in acetonitrile. Increased optical density of the sample is recorded after incubation at wave length being equal to 347.5-410.0 nm during 8-10 min. Enzyme activity is calculated from a formula. EFFECT: enhanced accuracy in determining elastase activity.
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