FIELD: medicine, immunology. SUBSTANCE: strain of E. coli ВКНМ B-5279 showing high level of polypeptide synthesis and property of human lymphotoxin is used as a source of the end product. After cultivation of strain-producer, cell rupture and separation of cellular residue two-stage chromatographic purification of extract is carried out (on DEAE-cellulose at pH 8.7-9.2 and hydroxyapatite at pH 6.6-6.8). EFFECT: high yield of homogenous human lymphotoxin preparation. 5 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING HUMAN RECOMBINANT TUMOR NECROSIS BETA-FACTOR | 1997 |
|
RU2132385C1 |
| RECOMBINANT PHAGE DNA M13 POL T7 AND RECOMBINANT STRAIN OF PHAGE M13 - A PRODUCER OF PHAGE T7 RNA-POLYMERASE | 1994 |
|
RU2089613C1 |
| METHOD FOR PREPARING RESTRICTION ENDONUCLEASE | 0 |
|
SU1634713A1 |
| METHOD FOR ISOLATION OF ENDONUCLEASE RESTRICTION APA 1 | 0 |
|
SU1655986A1 |
| METHOD OF PRODUCING ENDONUCLEASE OF NNA1 RESTRICTION | 0 |
|
SU1613490A1 |
| METHOD OF RESTRICTION ENDONUCLEASE PREPARATION, WHICH IS ABLE TO RECOGNIZE AND TO CLEAVE NUCLEOTIDE SEQUENCE G-T-C-G-A-C | 0 |
|
SU1752769A1 |
| STRAIN OF PARACOCCUS DENITRIFICANS BACTERIA AS PRODUCER OF ENDONUCLEASE OF RESTRICTION PDE 12 I | 0 |
|
SU1532583A1 |
| METHOD FOR PREPARATION OF RESTRICTION ENDONUCLEASE, RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5ЪTGATCA3Ъ | 0 |
|
SU1724689A1 |
| STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF KLENOW FRAGMENT | 1987 |
|
RU1602055C |
| DNA S54 FRAGMENT ENCODING POLYPEPTIDE SHOWING ACTIVITY OF GAMMA-INTERFERON, RECOMBINANT PLASMID DNA PROVIDING THE SYNTHESIS OF POLYPEPTIDE WITH ACTIVITY OF GAMMA-INTERFERON, AND THE STRAIN OF ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH GAMMA-INTERFERON ACTIVITY | 1992 |
|
RU2046144C1 |
