RECOMBINANT PHAGE DNA M13 POL T7 AND RECOMBINANT STRAIN OF PHAGE M13 - A PRODUCER OF PHAGE T7 RNA-POLYMERASE Russian patent published in 1997 - IPC

FIELD: biotechnology, genetic and protein engineering. SUBSTANCE: recombinant DNA M13polT7 is constructed by insertion of vector phage M13 mp 10 in DNA by unique restriction site Bam HI Sau- 3A-Sau 3A of plasmid pAR 1219 fragment containing phage T7 RNA-polymerase gene. On the base of the constructed recombinant DNA the strain of phage M13 VKPM pH-714 is obtained that provides synthesis of T7 DNA-polymerase at yield 2 x 10⁶ U/g wet biomass. EFFECT: increased yield of the end product. 2 cl, 2 dwg