METHOD OF PREPARING HUMAN RECOMBINANT TUMOR NECROSIS BETA-FACTOR Russian patent published in 1999 - IPC

Abstract RU 2132385 C1

FIELD: biochemistry, biotechnology. SUBSTANCE: method of isolation and purification of human recombinant tumor necrosis beta-factor involves culturing the strain-producer E. coli SG 20050/pLT21, ultrasonic cell disintegration, removal of cellular debris, chromatography purification on columns with DEAE-cellulose and hydroxyapatite and an additional stage of purification on hydroxyapatite in gradient concentration of potassium dihydrogen phosphate solutions at pH values 7.0-7.2. Method ensures to obtain the preparation of human recombinant tumor necrosis beta-factor free of impurities of foreign proteins, DNA and lipopolysaccharides and useful for medicinal aims. EFFECT: improved method of preparing, enhanced purity. 2 dwg, 4 tbl, 3 ex

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RU 2 132 385 C1

Authors

Sinichkina S.A.

Pustoshilova N.M.

Masycheva V.I.

Lebedev L.R.

Korobko V.G.

Dates

1999-06-27—Published

1997-04-10—Filed