STRAIN OF BACTERIUM CORYNEBACTERIUM PSEUDOTUBERCULOSIS USED AS REFERENCE ONE AND FOR PREPARING DIAGNOSTIC PREPARATIONS AT CASEOUS LYMPHADENITIS Russian patent published in 1996 - IPC

FIELD: biotechnology. SUBSTANCE: strain Corynebacterium tuberculosis ВГНКИ N209R-ДЕП is obtained for accelerated identification of caseous lymphadenitis pathogen at bacteriological, allergic and serological diagnosis of infection in sheep. Molecular properties of strain DNA: spectral data: A 260/280 = 1.9, A 260/230 = 2.3, concentration is 3333 mcg/ml, G + C content = 45.1 mole%. Based on homology nucleotide sequence of DNA this strain is concerned to the group II. For diagnosis allergen prepared from this strain is administrated in subtail wrinkle to sheep by intracutaneous route. Results are recorded after 24 hr. Inflammatory reaction appears in the place of diagnosticum administration in sick animals. This reaction is absent in health animals. Method ensures to detect additionally up to 80% infected animals as compared with conventional way (by palpation of surface lymphatic nodes). EFFECT: improved method of diagnosis.