FIELD: fish and meat industries, biotechnology and medicine. SUBSTANCE: muscle tissue is homogenized in 0.07- 0.15 N KCl solution at tissue to solution volumetric ratio of 1:1-1.5 at temperature of 0-4 C. The homogenate is extracted with 0.07-0.15 N KCl solution, the resulting precipitate is subsequently separated, the enzyme is purified from ballast proteins by affinic chromatography with intermediate dialysis and lyophilic drying is carried out. EFFECT: increased yield of the enzyme agent and simplified process.
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Authors
Dates
1996-08-27—Published
1995-09-12—Filed