FIELD: veterinary. SUBSTANCE: vaccine strain "ЭПМ" of carnivorous plague virus is used for preparing living cultural vaccine against carnivorous plague. Strain is grown on Eagle medium with KPC serum or with foetal serum and antibiotics. Repeated virus growing is carried out on serumless medium that ensures to decrease concentration of heterologic proteins up to 0.3 mg/ml "ВСЖ". 0.7 vol. p.p. "ВСЖ" at biological virus activity 103,7-0,2ТЦД50/мл is mixed with 0.3 vol. p.p. stabilizing agent that is a mixture of sorbitol, polyglucine and gelatose (0.05; 0.05 and 0.2 vol. p.p., respectively). Vaccine is poured into ampoules or small bottles (by 0.5 ml) and lyophilized. Vaccine can be used for prophylaxis against carnivorous plague. EFFECT: improved method of vaccine preparing. 2 tbl
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