METHOD OF LYSOZYME DIMER PREPARING Russian patent published in 1996 - IPC

Abstract RU 2067617 C1

FIELD: biotechnology. SUBSTANCE: method involves dimerization of lysozyme monomer using suberimidate cross-linking reagent in buffer solution at pH 10; dimerization ceasation at desired time by pH decrease to 7 by HCl or other acid addition; purification of dimerized lysozyme by the some successive elutions using ion-exchange column chromatography. Nondimerized lysozyme monomers after the first dimerization were recovered to process in order to increase the yield. Prepared lysozyme dimer can be used for treatment viral and/or bacterial diseases since it does not show cytotoxic effect as lysozyme monomer. EFFECT: increased yield, low price. 19 cl, 2 dwg

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RU 2 067 617 C1

Authors

Peter Kherrmann[Ch]

Peter Klajn[Ch]

Dates

1996-10-10—Published

1990-01-08—Filed