FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of hybrid protein in Escherichia coli cells, and can be used for purification of biotechnological substances from bacterial lipopolysaccharides. A hybrid protein P36GP12 consisting of an N-terminal oligopeptide MRGSHHHHHHGSAN and full-length protein of short tail filaments of T4-like bacteriophage isolate. Plasmid pQE-30_P36GP12_GP57 is designed, which provides synthesis of recombinant protein P36GP12 in Escherichia coli DH5αF'/pQE-30_P36GP12_GP57 strain cells, obtained by transformation of cells Escherichia coli DH5αF' constructed plasmid.
EFFECT: invention enables to obtain a recombinant protein P36GP12, having the ability to bind lipopolysaccharides Escherichia coli.
3 cl, 6 dwg, 6 ex
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