FIELD: biotechnology, gene engineering, pharmaceutical industry, medicine.
SUBSTANCE: method for production of recombinant placental growth factor (PLGF) includes culturing of modified prokaryotic cells containing inducible expression system of PLGF to produce OD600 of 14-50, followed by expression inducing, extraction, purification and solubilizing of inclusion bodies; enriching of obtained mixture with dimer a multimer form of expressed protein by anion exchange chromatography and isolation of PLGF preferably in dimmer form by reversed-phase chromatography.
EFFECT: method for PLGF production with high specific activity and high yield.
27 cl, 5 dwg, 5 ex
