RECOMBINANT PLACENTAL GROWTH FACTOR AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2007 - IPC C07K1/14 C07K14/475 C12N15/12 

Abstract RU 2295535 C2

FIELD: biotechnology, gene engineering, pharmaceutical industry, medicine.

SUBSTANCE: method for production of recombinant placental growth factor (PLGF) includes culturing of modified prokaryotic cells containing inducible expression system of PLGF to produce OD600 of 14-50, followed by expression inducing, extraction, purification and solubilizing of inclusion bodies; enriching of obtained mixture with dimer a multimer form of expressed protein by anion exchange chromatography and isolation of PLGF preferably in dimmer form by reversed-phase chromatography.

EFFECT: method for PLGF production with high specific activity and high yield.

27 cl, 5 dwg, 5 ex

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RU 2 295 535 C2

Authors

Mal'One Domeniko

Battisti Mauro

Konti Ehttore

Sal'Via Dzhuzeppe

Tuchchi Marina

Dates

2007-03-20Published

2002-02-05Filed