FIELD: veterinary medicine. SUBSTANCE: method involves obtaining in series pig growth hormone gen cDNA with N-end alanine segment, inserting it into plasmide useable for being expressed in Escherichia coli immediately after methionine codon used to initiate translation, transforming Escherichia coli strain with obtained recombinant plasmide, selecting transformed cells, cultivating the cells in appropriate culturing medium to accumulate the end product. Expressed polypeptide having no additional methionine residual at the N-end is subjected to separation and cleaning. EFFECT: enhanced effectiveness of treatment. 10 dwg, 2 tbl
Authors
Dates
1997-01-27—Published
1990-08-20—Filed