METHOD FOR OBTAINING RECOMBINATION POLYPEPTIDE POSSESSING GROWTH HORMONE PROPERTIES Russian patent published in 1997 - IPC

Abstract RU 2072393 C1

FIELD: veterinary medicine. SUBSTANCE: method involves obtaining in series pig growth hormone gen cDNA with N-end alanine segment, inserting it into plasmide useable for being expressed in Escherichia coli immediately after methionine codon used to initiate translation, transforming Escherichia coli strain with obtained recombinant plasmide, selecting transformed cells, cultivating the cells in appropriate culturing medium to accumulate the end product. Expressed polypeptide having no additional methionine residual at the N-end is subjected to separation and cleaning. EFFECT: enhanced effectiveness of treatment. 10 dwg, 2 tbl

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RU 2 072 393 C1

Authors

Gven Grabovskij Krivi[Us]

Dates

1997-01-27Published

1990-08-20Filed