FIELD: biochemistry, microbiology. SUBSTANCE: invention relates to method of preparing heterogeneous polypeptide in yeast cells Saccharomyces cerevisiae. Yeast is transformed with recombinant expressing vector comprising DNA construction encoding polypeptide that involves cross-linked signal peptide and leader peptide and heterogeneous polypeptide. Transformed yeast is cultured in suitable for expression medium and isolation of end product from cultural medium is carried out. Invention ensures to obtain the higher yield of correctly processed end protein. EFFECT: improved method of polypeptide preparing. 21 cl, 18 dwg, 2 tbl, 8 ex
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