METHOD OF CHEMICAL REAGENT TOXICITY DETECTION IN CULTURED HUMAN EMBRYO SKIN AND LUNG FIBROBLASTS Russian patent published in 1997 - IPC

FIELD: medicine, biology, medicinal toxicology. SUBSTANCE: method involves complex estimation of fibroblast metabolism normal alternation after effect with chemical reagents. The following indices were determined simultaneously for each sample: 1) activity of DT-diaphorase; 2) activity of lactic acid dehydrogenase; 3) cell resistance to the induced lipid peroxidation. Method can be used for testing medicinal and cosmetic preparations and for estimation of environment pollution. EFFECT: early detection of toxic effect.