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2 082 411

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C1

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IPC

(21) (22)

Application

93045359/14, 1993-09-15

(22)

Actual filing date

1993-09-15

(45)

Published

1997-06-27

(72)

Inventor

Antashev A.V.Shkurko V.I.

(73)

Holder

Nauchno-Issledovatel'Skij Institut Transplantologii I Iskusstvennykh Organov Mz Rf

METHOD OF PREPARING THE REAGENT FOR DETERMINATION OF HEMOLYTIC ACTIVITY OF THE FIRST COMPONENT COMPLEMENT IN HUMAN AND ANIMAL SERUM BLOOD Russian patent published in 1997 - IPC

Abstract RU 2082411 C1

FIELD: immunology. SUBSTANCE: the first component of complement is removed from serum blood by addition of polyethylene glycol (molecular mass is 6 kDa) at concentration 4.1-4.3%. After precipitate removing supernatant is diluted by two-fold with 4.1-4.3% of the same polyethylene glycol aqueous solution and acidified to pH = 5.5. All residual activity of C1 is transferred to precipitate. Then precipitate is removed by centrifugation and the obtained reagent is alkalinized to pH = 6.5. EFFECT: improved method of reagent preparing. 2 tbl

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RU 2 082 411 C1

Authors

Antashev A.V.

Shkurko V.I.

Dates

1997-06-27—Published

1993-09-15—Filed

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