FIELD: medicine, pediatry. SUBSTANCE: method involves enzymatic determination of antigens of pathogens of herpes, cytomegaly, toxoplasmosis, rubella and antibodies to their. Commercial diagnostics for RSK were combined with the analyzed material at equal volumes and 4 doses of complement were added. The presence of antibodies and antigen is determined by nonbound complement detected by catalase activity of the lyzed sheep erythrocytes. The own complement is removed from the analyzed material by sorption on neutral complement of hemolysin and sheep erythrocytes treated with 7.5-15% hydrogen peroxide followed by centrifugation. Method ensures to determine antibodies and antigen in the analyzed material quantitatively for 3-4 h. EFFECT: improved method of assay, accelerated analysis. 3 tbl
Authors
Dates
1997-09-10—Published
1995-04-24—Filed