METHOD OF PREPARING BACTERIAL FERMENT AND A METHOD OF PRODUCTION OF BACTERIAL CONCENTRATE AND ITS USE FOR FERMENTED-MILK FOODSTUFFS Russian patent published in 1997 - IPC

FIELD: biotechnology, food industry. SUBSTANCE: preliminary prepared symbiosis of lactic acid and acetic acid bacterium cultures is combined with symbiosis of bifidobacteria, propionic acid and acetic acid bacteria at ratio = 1:3. Milk is added at amount 1-5% and kept at 33-35 C for 8-16 h. For preparing bacterial concentrate bacterial ferment "Simbiter" (sowing material) is added to the nutrient medium at amount 3-5% and bacterial mass is accumulated at 33-34 C for 10-12 h. Dairy base for nutrient and protective media is fermented using combination of strains Streptococcus thermophilus VKPM B-4741, Lactococcus lactis ssp. cremoris VKPM B-4541 and Acetobacter aceti VKPM B-5495 taken at ratio = 2:2:1. EFFECT: enhanced effectiveness of ferment, increased live cells content in milk. 3 cl, 8 tbl