METHOD OF ISOLATION OF GLYCOSEAMINOGLYCANS FROM THE FIBROUS CARTILAGE Russian patent published in 1997 - IPC

FIELD: clinical biochemistry. SUBSTANCE: method involves proteolysis of the milled sample with papain, depoteinization of hydrolyzate with trichloroacetic acid and precipitation of glycoseaminoglycans with ethanol containing potassium or sodium acetate. Proteolysis is carried out at constant stirring sample in 0.05-0.1 M acetate buffer containing papain at amount 1/5 of crude tissue weight at +60 ± 4 C for 1.5-2 h. Deproteinization of the obtained hydrolyzate and glycoseaminiglycans precipitation with ethanol is carried out for 1.5-2 h at (-10)-(-20) C. EFFECT: accelerated method of isolation (by 2-3 days).