FIELD: medicine, cytology, biotechnology. SUBSTANCE: method involves tissue microfragments of diameter 0.5-0.8 mm. Ratio of culture tissue to bottle volume = 1: 3. Culturing is carried out at 20-26 C, at the rate of roller rotation 60-70 rev/min. Part of medium is exchanged by every 24 h. EFFECT: improved method of preparing.
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