FIELD: biology, cytology. SUBSTANCE: method involves using 0.1-0.2% collagenase solution containing 0.01-1% of DNase. Cells are cultured in DMEM medium containing 20% of calf embryo serum. Method provides preparing the monolayer of testicle primary cells enriched with Leidig's cells with viability 97-99%. EFFECT: improved preparing method. 1 tbl, 1 ex
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