FIELD: biology; agriculture.
SUBSTANCE: present invention relates to the field of cell biology, in particular to the isolation from the testes of boars of early sex cells of type A spermatogonia, purifying them by 99 % of somatic cells and maintaining in vitro. Claimed method allows to maintain in culture a long time of boar spermatogenies without signs of differentiation and to obtain a homogeneous gamete population, not contaminated with other cell types, for in vitro manipulation.
EFFECT: method can be used in agricultural biotechnology to obtain and preserve rare animals, as well as in veterinary virology.
1 cl, 1 dwg, 4 ex, 2 tbl
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Authors
Dates
2018-08-03—Published
2016-06-22—Filed