METHOD OF MAINTAINING IN A CULTURE OF BOAR SPERMATOGONIA TYPE A: SELECTION AND CULTIVATION IN VITRO Russian patent published in 2018 - IPC C12N5/00 C12N5/76 

Abstract RU 2663352 C1

FIELD: biology; agriculture.

SUBSTANCE: present invention relates to the field of cell biology, in particular to the isolation from the testes of boars of early sex cells of type A spermatogonia, purifying them by 99 % of somatic cells and maintaining in vitro. Claimed method allows to maintain in culture a long time of boar spermatogenies without signs of differentiation and to obtain a homogeneous gamete population, not contaminated with other cell types, for in vitro manipulation.

EFFECT: method can be used in agricultural biotechnology to obtain and preserve rare animals, as well as in veterinary virology.

1 cl, 1 dwg, 4 ex, 2 tbl

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RU 2 663 352 C1

Authors

Savchenkova Irina Petrovna

Vasileva Svetlana Aleksandrovna

Gulyukin Mikhail Ivanovich

Dates

2018-08-03Published

2016-06-22Filed