FIELD: veterinary biotechnology. SUBSTANCE: method involves DNA isolation from the examined microorganisms. DNA is cleaved with restriction endonuclease Nco I and hybridized with synthetic oligonucleotide probe. Method is used for differentiation of pathogenic and vaccine cattle brucella strains, for development of preparations used for diagnosis of cattle brucellosis. The known methods ensure to detect the species appliance of brucella only. EFFECT: improved method of differentiation. 1 dwg
Authors
Dates
1997-11-10—Published
1994-07-01—Filed