METHOD OF ISOLATION OF ALPHA-FETOPROTEIN FROM THE HUMAN SERUM BLOOD Russian patent published in 1997 - IPC

FIELD: biochemistry, biotechnology. SUBSTANCE: method involves the successive separation of the human serum blood by ion-exchange chromatography on column with DEAE-Sepharose equilibrated with 0.03-0.05 M potassium-phosphate buffer, pH = 6.1-6.3, immunoaffinity chromatography on column with Br-CN-activated Sepharose CL-4B bound with rabbit polyclonal antibodies raised to human alpha-fetoprotein, immunoaffinity chromatography on column with rabbit antibodies raised to human normal serum, immunoaffinity chromatography on column with rat antibodies raised to rabbit immunoglobulins followed by chromatography on column with Biogel AcA-34 and equilibrated with 0.004-0.006 M sodium-phosphate buffer, pH = 7.4-7.6. Eluting solution at immunoaffinity chromatography is 0.03-0.05 M potassium-phosphate buffer, pH = 6.1-6.3, containing 0.18-0.22 M sodium chloride. Immunoaffinity chromatography with rabbit polyclonal antibodies raised to the human alpha-fetoprotein is carried out with buffer solution 0.04-0.05 M Tris-HCl, pH = 7.8-8.0. The isolated and purified human alpha-fetoprotein can be used in medicine. EFFECT: improved method of isolation and purification. 1 dwg