FIELD: medicine.
SUBSTANCE: what is offered is a method for recovery and purification of a fragment of human alpha-fetoprotein. The method involves recovery of inclusion bodies containing a target protein from a producer strain biomass, solubilisation followed by renaturation and purification of the active fragment of human alpha-fetoprotein.
EFFECT: invention is applicable for producing the active fragment of human alpha-fetoprotein for the purpose of making anticancer preparations of directed action for selective tumour cell destruction.
5 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| ANTITUMOR PEPTIDE PREPARATION BASED ON ALPHA-FETOPROTEIN FRAGMENT, ITS CONJUGATE, PHARMACEUTICAL COMPOSITION AND METHOD FOR TREATMENT OF HORMONE-DEPENDENT TUMORS | 2005 |
|
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|
RU2404812C1 |
| ANTI-TUMOUR PREPARATION | 2011 |
|
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| Escherichia coli BL21(DE3)/pAFP11D3 STRAIN - PRODUCER OF 404 TO 609 AMINO ACID FRAGMENT OF HUMAN ALPHA-FETOPROTEIN | 2010 |
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|
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| METHOD OF PRODUCING CONJUGATE OF FRAGMENT OF HUMAN ALPHA-FETOPROTEIN WITH CIPROFLOXACIN | 2010 |
|
RU2431639C1 |
| METHOD OF PREPARING PREPARATION FOR DIRECTED DELIVERY OF ANTITUMOR DRUG IN CANCER CELL | 1998 |
|
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| METHOD FOR PRODUCTION OF PROTEIN PREPARATION BELONGING TO STRESS GENUS AND HSP70 PROTEIN PREPARATION OBTAINED BY SAID METHOD | 2005 |
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| PHOTOSENSITIZE BASED ON CARBOCYANINE DYE FOR PHOTODYNAMIC THERAPY OF TUMOURS | 2016 |
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| CONJUGATE OF BIOLOGICALLY ACTIVE SUBSTANCES WITH $$$-FETOPROTEIN SHOWING THE SELECTIVE ACTION WITH RESPECT TO CANCER TUMORS, METHOD OF THEIR PREPARING (VARIANTS) AND PHARMACEUTICAL COMPOSITION BASED ON THEREOF | 1996 |
|
RU2071351C1 |
