FIELD: medicine, neurohistology. SUBSTANCE: impregnation of histological preparation is carried out at 10-15 C and pH = 5.5-5.7 with solution containing the following components, wt.-%: manganese sulfate 3.04-3.14; cysteine 0.53-0.78, and water. Manganese is precipitated with 0.5-1.0% potassium ferrocyanide solution and precipitate is treated with 0.1-0.2% silver nitrate solution and 0.3-0.6% sodium sulfide solution. EFFECT: improved method of staining, increased degree of contrast and density. 2 dwg
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Authors
Dates
1998-01-27—Published
1993-03-31—Filed