METHOD FOR NEURONS AND THEIR AXONS IDENTIFICATION IN CENTRAL NERVOUS SYSTEM Russian patent published in 2017 - IPC G01N33/48 A61D99/00 

FIELD: medicine.

SUBSTANCE: invention relates to a method for neurons and their axons identification in the central nervous system, which includes flushing of investigated material vessels with 5% glucose solution, placement in a 10% solution of Na₂S, flushing with 5% glucose solution and distilled water, slices preparation and filling with balm, different in the fact that after investigated material vessels flushing with 5% glucose solution, 0.133 m CoCl₂ or CoSO₄ solution on 5% glucose is introduced for 15-20 minutes and after placement in the 10% solution of Na₂S washed out by the glucose solution, it is rinsed for 5-10 minutes in 10% formalin solution and the frozen slices are quickly (no more than 10 seconds) treated in 0.1% solution of AgNO₃, carefully washed in distilled water, with further dehydration and embalming, and black COS granules and fine axons and dark brown dendrites are detected in the studied neurons.

EFFECT: increased efficiency of detection of neurons, their axons and dendrites.

1 cl