FIELD: molecular biology, genetic engineering. SUBSTANCE: method involves an isolation of virion DNA, its cleavage with restricatse Bam HI, Sal I, Kpn I, separation of obtained DNA-fragments by electrophoresis in agarose gel and comparison restriction pattern with the known strains. For separation of Kpn I-split fragments pulse-electrophoresis is carried out additionally. Method ensures to differentiate and identify isolates and viral strains of African plague in pigs. EFFECT: high effectiveness of analysis. 1 dwg, 4 tbl, 1 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD AND SET FOR DETECTION OF AFRICAN PLAGUE PIG VIRUS DNA BY METHOD OF POLYMERASE CHAIN REACTION | 1996 |
|
RU2125089C1 |
METHOD OF TECHNOLOGICAL CONTROL OVER COMPLETENESS INACTIVATION OF THE KILLED VACCINES USING AS COPPER SULFATE AS INACTIVATING AGENT | 1995 |
|
RU2089893C1 |
SYNTHETIC OLIGONUCLEOTIDE PRIMERS, METHOD FOR DETECTING AND DIFFERENTIATING RIFT VALLEY VIRUS STRAINS BY POLYMERASE CHAIN REACTION AND RESTRICTION ENZYME DIGEST ANALYSIS | 2010 |
|
RU2457255C2 |
METHOD AND TEST SYSTEM TO DETECT DNA OF AFRICAN SWINE FEVER VIRUS BY MEANS OF SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION | 2007 |
|
RU2360971C1 |
RECOMBINATIONAL CARTRIDGE CONTAINING GENES EP153R AND EP402R OF STRAIN F-32 OF AFRICAN SWINE FEVER VIRUS AND RECOMBINANT STRAIN DSWCONGO/FRANCELECTINCD2 OF AFRICAN SWINE FEVER VIRUS | 2014 |
|
RU2571858C1 |
STRAIN "STAVROPOL 01/08" OF AFRICAN SWINE PLAGUE VIRUS FOR VIROLOGICAL, MOLECULAR-GENETIC AND MONITORING RESEARCH | 2010 |
|
RU2439152C1 |
STRAIN OF "KALUGA 2014" AFRICAN SWINE FEVER VIRUS FOR MONITORING RESEARCH AND STUDY OF PATHOGENESIS | 2015 |
|
RU2577997C1 |
ATTENUATED STRAIN "MSC-2015 VNIIVViM" OF AFRICAN SWINE FEVER VIRUS SEROTYPE VIII FOR VIROLOGY AND MOLECULAR-GENETIC ANALYSIS | 2016 |
|
RU2607791C1 |
METHOD FOR INDICATION AND DIFFERENTIATION OF VACCINE STRAINS AND FIELD ISOLATES OF CLASSIC HOG CHOLERA VIRUS | 2001 |
|
RU2196992C2 |
METHOD FOR DETERMINING ANTIGEN AFFINITY OF HEMABSORBING ISOLATES OF AFRICAN PIG PEST VIRUSES | 1996 |
|
RU2122211C1 |
Authors
Dates
1998-10-27—Published
1996-08-23—Filed