FIELD: medicine.
SUBSTANCE: primers are selected, and the PCR environment is optimised with amplification of gene NSs sequence fragments for the purpose of detecting a virus and pre-diagnosing Rift Valley fever. The PCR technology and restriction enzyme digest analysis are used to detect RNA in organ samples, including decomposed tissues of dead animals, in virus-containing cultural materials, veterinary inspection facilities, and differentiating the RVF virus strains and isolates.
EFFECT: method is to be included in the prevention program and epizootological monitoring, and is applicable in any diagnostic laboratory without damage of the involved personnel health.
2 cl, 2 tbl, 3 ex
