RECOMBINATIONAL CARTRIDGE CONTAINING GENES EP153R AND EP402R OF STRAIN F-32 OF AFRICAN SWINE FEVER VIRUS AND RECOMBINANT STRAIN DSWCONGO/FRANCELECTINCD2 OF AFRICAN SWINE FEVER VIRUS Russian patent published in 2015 - IPC C12N7/01 C12N15/63 

FIELD: biotechnologies.

SUBSTANCE: recombinant strain of the African swine fever virus with replacement of the genes EP153R and EP402R is offered which contains the reporter gene EGFP coding green fluorescent protein. The strain DswCongo/FranceLectinCD2 is obtained from the attenuated strain KK-262 of the African swine fever virus by homologous recombination. The strain comprises the genes EP153R and EP402R from the strain FK-32 of the African swine fever virus. The recombinant strain is not pathogenic for pigs at intramuscular infection in the dose 2*10⁶ GAE 50/cm³. The recombinational cartridge for obtaining of the recombinant strain DswCongo/FranceLectinCD2, which is a plasmid on the basis of the vector pUC19 containing fragments of the genes EP152R and EP364R of the strain KK-262 of the African swine fever virus, the full-size genes EP153R and EP402R of the strain F-32, and also the reporter gene EGFP is also offered.

EFFECT: invention can be used in scientific research institutes for study of role of proteins of the African swine fever virus in reproduction of the virus in cell cultures, in susceptible animals, and also their functions in modulation of immune response.

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