FIELD: biotechnologies.
SUBSTANCE: recombinant strain of the African swine fever virus with replacement of the genes EP153R and EP402R is offered which contains the reporter gene EGFP coding green fluorescent protein. The strain DswCongo/FranceLectinCD2 is obtained from the attenuated strain KK-262 of the African swine fever virus by homologous recombination. The strain comprises the genes EP153R and EP402R from the strain FK-32 of the African swine fever virus. The recombinant strain is not pathogenic for pigs at intramuscular infection in the dose 2*106 GAE 50/cm3. The recombinational cartridge for obtaining of the recombinant strain DswCongo/FranceLectinCD2, which is a plasmid on the basis of the vector pUC19 containing fragments of the genes EP152R and EP364R of the strain KK-262 of the African swine fever virus, the full-size genes EP153R and EP402R of the strain F-32, and also the reporter gene EGFP is also offered.
EFFECT: invention can be used in scientific research institutes for study of role of proteins of the African swine fever virus in reproduction of the virus in cell cultures, in susceptible animals, and also their functions in modulation of immune response.
2 cl, 2 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINATIONAL CARTRIDGE CONTAINING THE EP153R AND EP364R GENES OF THE CONGO (KK-262) STRAIN OF THE AFRICAN SWINE FEVER VIRUS AND RECOMBINANT ΔCONGOCD2V STRAIN OF THE AFRICAN SWINE FEVER VIRUS | 2016 |
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| STRAIN OF "KALUGA 2014" AFRICAN SWINE FEVER VIRUS FOR MONITORING RESEARCH AND STUDY OF PATHOGENESIS | 2015 |
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| ACHS/VNIIZZH/CV-1 STRAIN OF AFRICAN SWINE FEVER, WITH DECREASED VIRULENCE FOR PIGS, FOR VIROLOGIC, DIAGNOSTIC, MOLECULAR-GENETIC AND SURVEILLANCE STUDIES | 2018 |
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| STRAIN "STAVROPOL 01/08" OF AFRICAN SWINE PLAGUE VIRUS FOR VIROLOGICAL, MOLECULAR-GENETIC AND MONITORING RESEARCH | 2010 |
|
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| ATTENUATED ASFV/CV60/2020 STRAIN OF AFRICAN SWINE FEVER VIRUS OF THE ASFARVIRIDAE FAMILY, GENUS ASFIVIRUS FOR THE STUDY OF IMMUNOLOGICAL REACTIONS, MOLECULAR GENETIC ANALYSIS, GENETIC MODIFICATION AND CREATION OF A PROTOTYPE VACCINE AGAINST ASF | 2021 |
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RU2760399C1 |
| ATTENUATED STRAIN OF SEROTYPE 2 AFRICAN SWINE FEVER VIRUS FOR DEVELOPING DIAGNOSTIC AND VACCINE PREPARATIONS | 2010 |
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| ATTENUATED STRAIN "MSC-2015 VNIIVViM" OF AFRICAN SWINE FEVER VIRUS SEROTYPE VIII FOR VIROLOGY AND MOLECULAR-GENETIC ANALYSIS | 2016 |
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RU2607791C1 |
| METHOD OF DIFFERENTIATION AND IDENTIFICATION OF ISOLATES AND VIRAL STRAINS OF AFRICAN PLAGUE IN PIGS | 1996 |
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RU2121002C1 |
| RECOMBINANT VECTOR CONTAINING THE IMMUNOGENIC PROTEIN OF THE AFRICAN SWINE FEVER VIRUS, RECOMBINANT BACTERIA AND THEIR APPLICATION | 2020 |
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RU2771177C1 |
