FIELD: biotechnology, in particular E.coli O157:H7, O26:H11, O55:H7, O111:H8, O113:H21, and O117:H14 serotypes identification.
SUBSTANCE: claimed kit comprises immunomagnetic separating test-system and test-system for multiplex polymerase chain reaction (PCR). Immunomagnetic separating test-system contains carrier such as magnetic latex particles and sensitine, namely protein A covalently bound to rabbit specific immunoglobuline to polysaccharide fractions: LPS O157, O26, O55, O113, O111, O117 and to protein fagellar antigens: H7, H11, H8, H21, H14. Kit contains six groups of 0.5 % particle suspension of magnetic latex protein/polysaccharide complex. Test-system for PCR contains buffer with specific primers for enterohaemorragic E. coli stx1, stx2, rfb, fliC gene identification. Kit of present invention is useful in laboratory diagnosis of hemorrhagic colitis, etc., as well as for enterohaemorragic E. coli identification in foodstuffs.
EFFECT: kit of improved sensitivity and selectivity.
2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| DIAGNOSTICUM FOR IDENTIFICATION OF ESCHERICHIA COLI 0157:H7 AND METHOD OF ITS PREPARING | 2001 |
|
RU2189253C1 |
| SYNTHETIC OLIGONUCLEOTIDE KIT FOR IDENTIFYING HUMAN MONOCYTIC EHRLICHIOSIS Ehrlichia spp AGENT DNA BY REAL-TIME POLYMERASE CHAIN REACTION | 2009 |
|
RU2415947C1 |
| COMPLEX GENE- AND IMMUNODIAGNOSTIC TEST-SYSTEM FOR IDENTIFICATION OF CHOLERA VIBRIONS OF O1 AND O139 SEROGROUPS AND ESTIMATION OF THEIR VIRULENCE | 2009 |
|
RU2404257C1 |
| BACTERIOPHAGE Escherichia coli V32 STRAIN FOR IDENTIFICATION OF Escherichia coli BACTERIA SEROGROUP O157 | 2010 |
|
RU2425877C1 |
| METHOD TO DETECT AVAILABILITY OF BACTERIA Escherichia coli O157:H7 IN BIOLOGICAL AND FOOD SAMPLES BASED ON IMMUNODETECTION COUPLED WITH POLYMERASE CHAIN REACTION | 2014 |
|
RU2569196C1 |
| METHOD OF OBTAINING DIAGNOSTIC POLYVALENT SERUM FOR INTENTIVYING FLAGGELAR ANTIGEN Y. ESCHERICHIA COLI | 0 |
|
SU1338153A1 |
| STRAIN OF ESCHERICHIA COLI SEROVAR O26:H11 BACTERIA, PRODUCER OF A SHIGA-LIKE TOXIN, DEPOSITED IN THE STATE COLLECTION OF PATHOGENIC MICROORGANISMS AND CELL CULTURES "SCPM-OBOLENSK" UNDER NUMBER B-8034 | 2020 |
|
RU2756980C1 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF SHIGA-LIKE TOXIN 2 (STX2) | 2023 |
|
RU2816500C1 |
| METHOD FOR PREPARING COMPOUNDS, COMPOSITION FOR PREPARING MEDICINAL AGENT | 2001 |
|
RU2290409C2 |
| APPLICATION OF BOVINE LACTOFERRIN FOR PREPARING MEDICINAL AGENT FOR BACTERIA GROWTH INHIBITION | 2006 |
|
RU2399380C2 |
