FIELD: genetics. SUBSTANCE: invention aims at extending spectrum of initial forms of selection through artificial creation of new mutant plant species. For that, acridine orange or ethydium bromide is added to nutrient mixture with exogenous DNA. Mixture is matured for 1-2 min and applied on pistil stigma: ethydium bromide to final concentration 10-5 M and acridine orange to final concentration 5•10-5 M. EFFECT: simplified procedure and increased transformation efficiency through pollination-fertilization step. 2 cl, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| PLANT TRANSFORMATION METHOD | 1997 |
|
RU2123781C1 |
| METHOD OF PLANT GENETIC TRANSFORMATION | 0 |
|
SU1708849A1 |
| METHOD OF TRANSFORMATION OF PLANT MULTIPLYING BY POLLINATION | 1988 |
|
RU2054482C1 |
| METHOD OF OBTAINING TRANSGENIC SORGHUM PLANTS | 2002 |
|
RU2229793C1 |
| POLYNUCLEOTIDE, METHODS OF PLANT PRODUCING, PLANT GENOME, CELLS, FRUITS, SEEDS, METHODS OF SEEDS PRODUCING, OLIGONUCLEOTIDE USING, PLASMID, MICROORGANISM | 1992 |
|
RU2170255C2 |
| METHOD FOR RETAINING RADIATED SEED PROMOTION EFFECT | 1997 |
|
RU2112346C1 |
| METHOD OF SELECTING TRANSFORMS OF TOMATOES | 1992 |
|
RU2037289C1 |
| WATER SWEETENING PROCESS | 1997 |
|
RU2120415C1 |
| DNA MOLECULE ENCODING PROTEIN ELICITING N-ACETYLPHOSPHINOTRICINE DEACETYLASE ACTIVITY AND METHOD FOR PREPARING PLANTS WITH PARTS DECOMPOSED BY INDUCTION | 1997 |
|
RU2205219C2 |
| METHOD OF OBTAINING TRANSGENIC MONOCOTYLEDON PLANTS | 2007 |
|
RU2351121C1 |
