FIELD: plant biotechnology. SUBSTANCE: method involves isolation of unmature pollen grains at the stage of mononucleus microspore either in the process of pollen method or at the stage of binucleus microspore as long as generative cell sticks to the pollen wall. Then adjacent tissue is removed and exogenic DNA is incorporated into unmature pollen grains placed in nutrient medium. Grains were carried out in vitro up to the complete maturation and plant-recipients were pollinated with transformed pollen grains. EFFECT: improved method of transformation.
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