METHOD FOR PREDICTING CHILDREN DIPHTHERIA INFECTION DEVELOPMENT COURSE Russian patent published in 2000 - IPC

FIELD: medicine. SUBSTANCE: method involves determining pharyngeal mucosa resistance to microbe action using epitheliocytes in brush mucosa biopsy samples. The epitheliocytes suspension in phosphate salt buffer having 7.2-7.4 pH value is produced with 0.5-0.7 billion, one day old cell culture of Corynebacterium diphtheriae added. The suspension is placed over two microscope slides after washing and holding it in thermostat at 37 C during 30 min. Adhesion factor is determined on one of the slides as micro-organism adhesion-resistant epitheliocyte percentage with respect to adhesion index as mean number of cells attached to one epitheliocyte. Specific luminescence is examined on the other slide witnessing secretory IgA availability. Adhesion factor being found to be greater than 10, adhesion index being less than 50 and IgA being available, favorable development course and issue of the infectious process is to be predicted. EFFECT: accelerated and cheap treatment method. 1 tbl