FIELD: biochemical methods. SUBSTANCE: plant tissue is treated with composition containing glutaric aldehyde and, as additional component, caffeine (0.5-1.5%) in Na-phosphate buffer and allowed then to stand for 3 h at room temperature. After that, tissue is washed out with the same buffer plus caffeine and fixed during 1 h with osmium tetraoxide solution, dehydrated with aqueous ethanol and acetone solutions, and packed. EFFECT: preserved native localization of phenol compounds in electron-optical examination of tea-leaf tissues and improved electron- optical image. 2 dwg, 3 ex
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Authors
Dates
2000-03-20—Published
1998-06-23—Filed