FIELD: medicine.
SUBSTANCE: method of infertility diagnostics for men involves ejaculate dissolution by isotonic NaCl solution, application of a drop of fixation agent with sediment cells suspended in it onto a piece of aluminium foil degreased preliminarily, and incubation. Afterwards the foil with attached cells is flushed in osmic acid with further fixation by filling into epoxy resin in flat trays and separation of aluminium foil from solid resin after polymerisation. Capsules are examined by photo-optical microscope. Fixated spermatozoids for ultrastructural examination are selected. Centrioles are examined, and conclusion on infertility is drawn by centriole component structure flaws, such as microtube triplet fragmentation or disorder of parallel orientation of triplets.
EFFECT: assessment of fertilising capacity of spermatozoids.
3 dwg
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