METHOD FOR PREPARING BLOOD PREPARATION FOR ELECTRON-MICROSCOPIC INVESTIGATION Russian patent published in 2006 - IPC G01N33/49 G01N1/06 G01N1/28 

FIELD: medicine, laboratory investigations.

SUBSTANCE: method for preparing a blood preparation for electron-microscopic investigation involves centrifugation of suspension of non-fixed isolated cells at the rate 1500 rev/min, removal of supernatant, resuspending a precipitate in 0.25 ml of 10% human serum albumin followed by addition one drop of 25% glutaric dialdehyde to the prepared mixture and centrifugation up to preparing gel. Prepared gel is poured with 10% formalin in phosphate buffer, pH 7.5, for 24 h followed by separation of gel for segments. Gel segments are subjected for additional fixation in 2% solution of osmium tetraoxide, dehydrated, poured in epoxy resins, slices are prepared, stained and contrasted. Method allows significant improving quality of the preparation.

EFFECT: improved preparing method.

2 cl, 3 dwg