METHOD OF BIOPREPARATION PREPARING Russian patent published in 2000 - IPC

FIELD: biotechnology, microbiology. SUBSTANCE: industrial strain of Bacillus subtilis is cultured in nutrient medium providing spore formation. Biomass is separated from nutrient medium, mixed with stabilizing agent based on sugar-gelatin medium and calcium glucanate taken in the amount 0.1-5.0 wt.% or with acetylphthalyl-cellulose, Bimass and stabilizing agent are taken at the ratio = (1:1)-(1:1.5). Obtained mixture is dried and placed into gelatin capsules. Nutrient medium has the following components, g/l: potassium dihydrogen phosphate, 0.2-0.4; ammonium sulfate, 1.0-3.0; sodium citrate, 1.0-3.0; copper sulfate, 0.001-0.01; zinc sulfate, 0.002-0.005; iron sulfate, 0.0001-0.001; calcium chloride, 0.09-0.2; magnesium sulfate, 0.1-0.5; manganese sulfate, 0.01-0.1; peptone, 2.0-8.0; maltose, 1.0-5.0; and water, the balance. After mixing biomass with acetylphthalylcellulose obtained microgranules are separated, washed out with physiological solution and drying is carried out without preliminary freezing. Obtained preparation shows enhanced antagonistic activity with respect to some microorganisms and enhanced adaptability of its bacteria in human gastroenteric tract. Invention can be used in production of curative-prophylactic preparations, in part, probiotics based on bacteria of genus Bacillus. EFFECT: improved method of biopreparation preparing. 4 cl, 3 ex