METHOD OF PRODUCING ANTIBIOTIC ACTINOPLACIN, STRAIN STREPTOMYCES (KITASATOA) SPECIES 834 VNIISKHM D-484 AS PRODUCER OF ANTIBIOTIC ACTINOPLACIN Russian patent published in 2000 - IPC

FIELD: biotechnology, production of antibiotics. SUBSTANCE: method involves culturing the novel strain-producer Streptomyces (Kitasatoa) species 834 VNIISKHM D-484. Strain is grown in nutrient medium containing glucose, dried yeast extract, calcium carbonate at 27 ± 1 C. Antibiotic from biomass is extracted with 65-70% aqueous acetone. Extracts are evaporated and antibiotic is purified by column chromatography using molecular sorbent based on copolymer of styrene and divinylbenzene. Desorption of antibiotic from column is carried out with 70% aqueous acetone. Isolation is carried out by precipitation with acetone or by evaporation followed by washing out with anhydrous acetone and drying under vacuum. New strain shows enhanced activity with respect to gram-positive and gram-negative bacteria. EFFECT: simplified technology of isolation, enhanced biological activity. 3 cl, 4 tbl, 3 ex