FIELD: biochemistry, enzymology. SUBSTANCE: invention relates to technology of enzyme production. Method of purification of ribonuclease involves dissolving and filtration of ribonuclease industrial salted out fraction. Filtrate is sorbed on isoporous macro-netted sulfocation-exchange resin that is a copolymer of styrene and monochloroethyl ester, pH = 4.5-6.5, followed by enzyme elution. For elution 30-60% ethanol or isopropanol in 0.1-0.5 N HCl is used. EFFECT: decreased time of technological cycle of ribonuclease purification, increased yield, improved quality of preparation. 2 cl, 1 dwg, 1 tbl, 3 ex
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